Insertion junction: LMJ.RY0402.142413_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.142413
Chromosome:chromosome 11
Location:337989
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467569 ATPD,ATPD1 Chloroplast ATP synthase delta chain; (1 of 1) K02113 - F-type H+-transporting ATPase subunit delta (ATPF1D, atpH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACAATTTTTGTGGCGCCGCTCCCAACG
Internal bar code:CCCTGCGACCCCTTGACTCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:585
LEAP-Seq percent confirming:98.9848
LEAP-Seq n confirming:195
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGATTTGACCTCGTTCGTGT
Suggested primer 2:ATTGACTCCAGCCTGATTGC