| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142440 |
| Chromosome: | chromosome 1 |
| Location: | 6332210 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045150 | FAP304 | Flagellar Associated Protein 304; (1 of 5) PTHR10351//PTHR10351:SF42 - TRANSCRIPTION FACTOR BTF3 // LD40663P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGAGGGAGGCAGGGCGAGCGGGGGGC |
| Internal bar code: | TACAGTTGGTCTTACGGGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 89.6573 |
| LEAP-Seq n confirming: | 5756 |
| LEAP-Seq n nonconfirming: | 664 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACAATGTAGGGGCATCG |
| Suggested primer 2: | TCGTTACAGCCCCTACATCC |