| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.142477 |
| Chromosome: | chromosome 10 |
| Location: | 2931925 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440350 | (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGGCTTCCGGCAGAGGAGACTATGGTG |
| Internal bar code: | CTAACTATTCTGGTGGAGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1037 |
| LEAP-Seq percent confirming: | 99.0975 |
| LEAP-Seq n confirming: | 549 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTCCACGTCTGTCAAG |
| Suggested primer 2: | CTGAGCTAGCTTCCACCCAC |