Insertion junction: LMJ.RY0402.142490_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.142490
Chromosome:chromosome 7
Location:4123221
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g342850 ODA5,DCC3 (1 of 2) PTHR21694//PTHR21694:SF18 - UNCHARACTERIZED // COILED-COIL DOMAIN-CONTAINING PROTEIN 114; Outer Arm Dynein assembly protein 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAGACACAGCCCCGCGGCCGCTGTCGTG
Internal bar code:TTCTGCCTCGTCGGCGGTAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:551
LEAP-Seq percent confirming:95.4493
LEAP-Seq n confirming:1657
LEAP-Seq n nonconfirming:79
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTTGTCTCCTCCCTCTG
Suggested primer 2:CAGTTGACAACCCACGTCAC