| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.142491 |
| Chromosome: | chromosome 1 |
| Location: | 5145545 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035800 | IRK2 | (1 of 2) PTHR11767//PTHR11767:SF47 - INWARD RECTIFIER POTASSIUM CHANNEL // SUBFAMILY NOT NAMED; Inwardly-rectifying potassium channel | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGAAGGCTGCCTGTTGAGTTTGCGCTG |
| Internal bar code: | GGTGCGTAAGGGCGTCGCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 454 |
| LEAP-Seq percent confirming: | 95.1168 |
| LEAP-Seq n confirming: | 448 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCGTGGTAAATGTCCTT |
| Suggested primer 2: | CCGGCACCAGTACAACTTTT |