Insertion junction: LMJ.RY0402.142502_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.142502
Chromosome:chromosome 2
Location:2340471
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g090750 (1 of 1) PF08574 - Protein of unknown function (DUF1762) (DUF1762) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGACGGCGCGTTGGCGCGGAAGGATCT
Internal bar code:GCGTGGTCGGCTTTGTAGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:792
LEAP-Seq percent confirming:99.9024
LEAP-Seq n confirming:3072
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGGTCAACAGAGGGGTG
Suggested primer 2:GACTACAGCGAGCACAGCAG