Insertion junction: LMJ.RY0402.142524_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.142524
Chromosome:chromosome_2
Location:7347709
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g145100 FAP39 P-type ATPase/cation transporter, flagellar associated sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTATCGCCGAACCCCTGCCCTTACGCCCCC
Internal bar code:TCTGTAAGCGGAGTCCTCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:864
LEAP-Seq percent confirming:99.5823
LEAP-Seq n confirming:4053
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:ACGACACAAACACACACGGT
Suggested primer 2:AAGACCAACCTCACCCACAG