Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.142524 |
Chromosome: | chromosome_2 |
Location: | 7347709 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g145100 | FAP39 | P-type ATPase/cation transporter, flagellar associated | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTATCGCCGAACCCCTGCCCTTACGCCCCC |
Internal bar code: | TCTGTAAGCGGAGTCCTCGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 864 |
LEAP-Seq percent confirming: | 99.5823 |
LEAP-Seq n confirming: | 4053 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGACACAAACACACACGGT |
Suggested primer 2: | AAGACCAACCTCACCCACAG |