Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.142525 |
Chromosome: | chromosome 2 |
Location: | 5197308 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCATCGCTACAGTAAGTCTTTTCGCAG |
Internal bar code: | GCGTCGCTGAACGGACCCGCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 881 |
LEAP-Seq percent confirming: | 93.8722 |
LEAP-Seq n confirming: | 720 |
LEAP-Seq n nonconfirming: | 47 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTTTGGCTATGTGCCTGTT |
Suggested primer 2: | TCTTTCACAACCAGGGTTCC |