Insertion junction: LMJ.RY0402.142525_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.142525
Chromosome:chromosome 2
Location:5197308
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTCATCGCTACAGTAAGTCTTTTCGCAG
Internal bar code:GCGTCGCTGAACGGACCCGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:881
LEAP-Seq percent confirming:93.8722
LEAP-Seq n confirming:720
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTTGGCTATGTGCCTGTT
Suggested primer 2:TCTTTCACAACCAGGGTTCC