Insertion junction: LMJ.RY0402.142532_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.142532
Chromosome:chromosome_1
Location:7241596
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre01.g052150 ANK2,FAP87 Flagellar Associated Protein with ankyrin repeats antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CACGTGCAGGTCAATCAGGCAGAGAGCGCC
Internal bar code:CCTTCTCCAGTGGGGTAGCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:749
LEAP-Seq percent confirming:99.5984
LEAP-Seq n confirming:992
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTAGAGGTATCGTGGCGG
Suggested primer 2:CAGAGTCAGTCGTGTCGCTC