| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.142532 |
| Chromosome: | chromosome_1 |
| Location: | 7241596 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g052150 | ANK2,FAP87 | Flagellar Associated Protein with ankyrin repeats | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCCACAACCGCAGCTACCGCACGCACTTT |
| Internal bar code: | CTCTATTTTCGATGTTTACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 99.0805 |
| LEAP-Seq n confirming: | 3879 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAGAGGTATCGTGGCGG |
| Suggested primer 2: | CAGAGTCAGTCGTGTCGCTC |