| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142533 |
| Chromosome: | chromosome 2 |
| Location: | 6805287 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118801 | GTR | Glycosyl transferase; (1 of 1) 2.4.1.246 - Mannosylfructose-phosphate synthase / MFPS | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTGCAGCTGCTGCCTCGTTCGTCTCG |
| Internal bar code: | AGGTACCGTCGCACGAGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 99.6743 |
| LEAP-Seq n confirming: | 1224 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGCAACAGCAGGGATAG |
| Suggested primer 2: | AAGATTTGATTGGGTGCTGC |