| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.142534 |
| Chromosome: | chromosome 3 |
| Location: | 8942031 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g208721 | CGL92 | (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor; predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGAAGGCGTAGCCAGGAGGGGGGGGAG |
| Internal bar code: | CCAGAGACGATCGTGTCCCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGAGCTGCTAGACGACG |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |