| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142548 |
| Chromosome: | chromosome 17 |
| Location: | 3675526 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726750 | SHK1,SHKA1,DHAPS1 | (1 of 1) 2.5.1.54 - 3-deoxy-7-phosphoheptulonate synthase / Phospho-2-oxo-3-deoxyheptonate aldolase; DHAP synthetase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATGTTTTAGCGGTGTTAGTGCGTCAAG |
| Internal bar code: | GGATTCAGGGATAGGCGCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 91.9526 |
| LEAP-Seq n confirming: | 3805 |
| LEAP-Seq n nonconfirming: | 333 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGGGTTAGGGGAATAGA |
| Suggested primer 2: | TGAGCAAGAGGGTGTGAGTG |