Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.142570 |
Chromosome: | chromosome_7 |
Location: | 1009499 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g319350 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGTTGATGCTGACTGCAACTCTGCAACCG |
Internal bar code: | GAGTACTATATAAGGGGCTTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 136 |
LEAP-Seq percent confirming: | 96.0714 |
LEAP-Seq n confirming: | 269 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTTGCTAGTTGCTTCCCAG |
Suggested primer 2: | AACTCCACCAAGAGCCAGAA |