Insertion junction: LMJ.RY0402.142588_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.142588
Chromosome:chromosome 6
Location:7367096
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g299150 PHC9,PHC26 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 26 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGTTTGACCAGCCCACGGACGGCCCCGC
Internal bar code:TATATAGGCCCCAGGATCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:251
LEAP-Seq percent confirming:99.6709
LEAP-Seq n confirming:5148
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGGGCAACAGCCAATAC
Suggested primer 2:AGGAGGAGGTGAAGGAGGAG