| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142611 |
| Chromosome: | chromosome 7 |
| Location: | 1179180 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g320850 | (1 of 2) 3.2.1.21 - Beta-glucosidase / Gentobiase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAACGTGATCTGCTGGTGCACCAGCCCC |
| Internal bar code: | GGTGGTGCAGCAGGGGGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 923 |
| LEAP-Seq percent confirming: | 99.8708 |
| LEAP-Seq n confirming: | 773 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCAAGGACACAGGTTCA |
| Suggested primer 2: | ATCCCAGGAAACAGTATGCG |