| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142623 |
| Chromosome: | chromosome 3 |
| Location: | 1184024 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149450 | (1 of 1) IPR006037//IPR016040 - Regulator of K+ conductance, C-terminal // NAD(P)-binding domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATGCCAGTAGTCTAAGAACTGCGACAA |
| Internal bar code: | GAATGCGAGTCCGTGAGGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 99.0909 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAGTCATCCAGACAGTT |
| Suggested primer 2: | TGGAGCATAGTCACGCACTC |