| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142625 |
| Chromosome: | chromosome 9 |
| Location: | 7938076 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g417350 | Macrocin O-methyltransferase; (1 of 1) 2.1.1.285 - Demethyldecarbamoylnovobiocin O-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTATGAGGAAGCAGGTCTTCTGCAGGTCT |
| Internal bar code: | GTAGATTCTTTCACAGGCAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 99.9425 |
| LEAP-Seq n confirming: | 1739 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTGCAACTGACCAAACC |
| Suggested primer 2: | AACAAAGGTATGCCAGGTGC |