Insertion junction: LMJ.RY0402.142632_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.142632
Chromosome:chromosome 10
Location:1053572
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g425251 (1 of 5) IPR008274 - Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGCCAGGGCGGCCCCGTCCTCGCCCGTG
Internal bar code:ATCGCAGGAGATGAACTGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:396
LEAP-Seq percent confirming:99.8132
LEAP-Seq n confirming:5344
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCCTCTGCTTCCTTCTTG
Suggested primer 2:CAACCAGGCAGTCCTCTAGC