| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142657 |
| Chromosome: | chromosome 16 |
| Location: | 6065162 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676200 | (1 of 3) PTHR10751:SF2 - GUANYLATE-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGCTAGTGATGTCAGACTGCTTTTCT |
| Internal bar code: | CCTCGATGGGGACTGATATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCACACACCAGCAGTA |
| Suggested primer 2: | TATAGGCGGCAGTGGTATCC |