| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142688 |
| Chromosome: | chromosome 6 |
| Location: | 2118765 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265550 | CDPK12 | (1 of 1) PF07714//PF13202 - Protein tyrosine kinase (Pkinase_Tyr) // EF hand (EF-hand_5); Calcium/calmodulin-dependent protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAAATGTGGACTACAACGAGTGGCTGG |
| Internal bar code: | CCTGCGGGTCACAAGCAACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 99.226 |
| LEAP-Seq n confirming: | 641 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACAACTCCTCCTGGCTC |
| Suggested primer 2: | TATTCAAGGTCCAAGCCAGG |