Insertion junction: LMJ.RY0402.142721_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.142721
Chromosome:chromosome 8
Location:3558596
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g375550 (1 of 25) IPR013216//IPR029063 - Methyltransferase type 11 // S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTTTACTCCCTTTCAAAACGCAGGCGCG
Internal bar code:TTGTAACCGTGGCCGATGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:629
LEAP-Seq percent confirming:91.6398
LEAP-Seq n confirming:3409
LEAP-Seq n nonconfirming:311
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTACTCGAAGCCCTTCTT
Suggested primer 2:ACCAACTTTGTCAAAACCGC