Insertion junction: LMJ.RY0402.142737_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.142737
Chromosome:chromosome 12
Location:4515866
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g521950 (1 of 5) K14347 - solute carrier family 10 (sodium/bile acid cotransporter), member 7 (SLC10A7, P7); Sodium:bile acid symporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTCTTGCCACAAATCACGTCCTGGAGCG
Internal bar code:GAGCCATGCCAGCGGGAATTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:391
LEAP-Seq percent confirming:99.7321
LEAP-Seq n confirming:2234
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GATGAGGGCTCCGGTACATA
Suggested primer 2:GAGTCAGCAAGGGTCAGGAG