| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142749 |
| Chromosome: | chromosome 14 |
| Location: | 359035 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610300 | (1 of 88) 3.6.4.12 - DNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATGTGTGCGCCCGGGCCAGTCGGATGT |
| Internal bar code: | GGCCTGTCGTGCGACATTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 869 |
| LEAP-Seq percent confirming: | 99.4595 |
| LEAP-Seq n confirming: | 2576 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACAGGAGGAGGTG |
| Suggested primer 2: | TACTCACAGCTTCGCCACAC |