Insertion junction: LMJ.RY0402.142762_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.142762
Chromosome:chromosome 7
Location:2382109
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g328500 ARS17 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCAGTCCGCCGGCTACAACACATACCT
Internal bar code:TACGTGTCTGCTCTGTAACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:427
LEAP-Seq percent confirming:99.5316
LEAP-Seq n confirming:5100
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTTACACGGAGGTGGAT
Suggested primer 2:AACTCTTCTCCTCGCCTTCC