| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142766 |
| Chromosome: | chromosome 2 |
| Location: | 4732129 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102100 | SMM5 | (1 of 2) PF13679 - Methyltransferase domain (Methyltransf_32); S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGAGCTGACTATTCGTGCAAAGGTAC |
| Internal bar code: | CGGGGGGCGGCGGCTTAAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 263 |
| LEAP-Seq percent confirming: | 99.6748 |
| LEAP-Seq n confirming: | 613 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACCCCTCCTGTCCTACA |
| Suggested primer 2: | GCTACGGTGTATGATGCCCT |