Insertion junction: LMJ.RY0402.142788_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.142788
Chromosome:chromosome 1
Location:6972631
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGCAAGCAAGCGCGCGGCGCGCACCACG
Internal bar code:GTTTTTGTAGATCTCCTGCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1193
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGGACCCAACAACATGTACC
Suggested primer 2:GAGCAGCACTCATCGTGGTA