Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.142837 |
Chromosome: | chromosome 9 |
Location: | 3472997 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g389504 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCCCTGGCCCATACACCCCAGCATTGA |
Internal bar code: | CAGTGGCGTCTTGACCGTAGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 185 |
LEAP-Seq percent confirming: | 56.1036 |
LEAP-Seq n confirming: | 455 |
LEAP-Seq n nonconfirming: | 356 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTAAGGGGGTGTCTGGTGAG |
Suggested primer 2: | GCCCTGTGTGATCATGTGTC |