Insertion junction: LMJ.RY0402.142851_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.142851
Chromosome:chromosome 16
Location:1357875
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g651900 CRI1,OPR129,OPR126,CRTISO OctotricoPeptide Repeat protein 129 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGCTGTCAAAGTCGTCAGCTGCATCCA
Internal bar code:CGGCGGTCACTACGAAGCTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:952
LEAP-Seq percent confirming:99.3199
LEAP-Seq n confirming:8032
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:AACACAACTGTTGCAGTGGC
Suggested primer 2:AGCCAACCAAAACGAAGCTA