Insertion junction: LMJ.RY0402.142910_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.142910
Chromosome:chromosome 3
Location:4202449
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g174050 MRP4 (1 of 3) PTHR24223//PTHR24223:SF252 - FAMILY NOT NAMED // PROTEIN MRP-2; ABC transporter, multidrug resistance associated protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGGCGCCGGCACCATCGCCAACCTGCAG
Internal bar code:TTCTTGACGTACTTTGAAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:282
LEAP-Seq percent confirming:99.7156
LEAP-Seq n confirming:1052
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACCCTCACACACACA
Suggested primer 2:TCTCAACATTACTCGCACGC