| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142936 |
| Chromosome: | chromosome 11 |
| Location: | 2098939 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469187 | (1 of 98) IPR023214 - HAD-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCACGCGGCTGGTTGCACCGTGTGCGT |
| Internal bar code: | CGCCAAAGCGCTTGAGGCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 97.7058 |
| LEAP-Seq n confirming: | 1448 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTATTAGCTTAGCACGCGG |
| Suggested primer 2: | TGGAGTTGAAGAACTGCGTG |