Insertion junction: LMJ.RY0402.142955_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.142955
Chromosome:chromosome 9
Location:1846152
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g395750 (1 of 6) 3.6.3.43 - Peptide-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCTCGTTGAGGCGCTCGAAAACCTTCT
Internal bar code:GCGCGGTACGCCCTCCTGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:834
LEAP-Seq percent confirming:86.7954
LEAP-Seq n confirming:9577
LEAP-Seq n nonconfirming:1457
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCTCGGTCTGGCTCACAT
Suggested primer 2:CAACGTGGTGGTTCATTGAG