Insertion junction: LMJ.RY0402.142959_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.142959
Chromosome:chromosome 7
Location:5702039
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g352600 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCACCCCGCCTCATCCCCACCCCAAAC
Internal bar code:CGTATTGAGCAGGGCCATTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:886
LEAP-Seq percent confirming:75.0252
LEAP-Seq n confirming:1487
LEAP-Seq n nonconfirming:495
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACACTCCCAGATCCTTG
Suggested primer 2:TCGTGTATGGCAGTGATGGT