| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142968 |
| Chromosome: | chromosome 12 |
| Location: | 4807463 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524400 | (1 of 1) IPR003128//IPR007122//IPR029919 - Villin headpiece // Villin/Gelsolin // Protein flightless-1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACTGGCGCCCTTTAATTCTATCTCGAGG |
| Internal bar code: | AGGTTTCTTCGAAGGACATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 425 |
| LEAP-Seq percent confirming: | 99.9589 |
| LEAP-Seq n confirming: | 2430 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCGCGTTACATACCAAA |
| Suggested primer 2: | TTGATGGGTCCCAATGAAAT |