| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142972 |
| Chromosome: | chromosome 13 |
| Location: | 2525474 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580500 | (1 of 2) PTHR12847:SF3 - NECAP-LIKE PROTEIN CG9132 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTGGTTTGCAGCGGAACTGTTTGCGGA |
| Internal bar code: | CCGGCTGTTCACTGGGATGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 840 |
| LEAP-Seq percent confirming: | 98.2859 |
| LEAP-Seq n confirming: | 2867 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCTGTGAAACCATCTCA |
| Suggested primer 2: | TGACACTACTGCCAAGACGC |