| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142985 |
| Chromosome: | chromosome 11 |
| Location: | 402315 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467580 | FAP370 | Flagellar Associated Protein 370 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGGCGTGCCGTTGAGGGTGGCGCTGCT |
| Internal bar code: | CCATGTGAAAATCTGTATAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 420 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCCCATAGCTCCGTGAT |
| Suggested primer 2: | GTTGTCGGTTTCCGTCTTGT |