| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142992 |
| Chromosome: | chromosome 2 |
| Location: | 981015 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCACTGACAGGACTGCGTCTGTGCAGC |
| Internal bar code: | GCTTTTTGCCCATTCAGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 90.463 |
| LEAP-Seq n confirming: | 6858 |
| LEAP-Seq n nonconfirming: | 723 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTAGAAGCATTGGAGGC |
| Suggested primer 2: | ACTGGGTTCGGTTCAGTTTG |