| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143022 |
| Chromosome: | chromosome 2 |
| Location: | 6372153 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115400 | (1 of 2) IPR002889//IPR008979 - Carbohydrate-binding WSC // Galactose-binding domain-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCATGCGGAAGTAGCCGAACACGCGCGC |
| Internal bar code: | TTGTATCTCGGGCTTAGGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1048 |
| LEAP-Seq percent confirming: | 99.432 |
| LEAP-Seq n confirming: | 7703 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCGTCCTTTGTAGCATC |
| Suggested primer 2: | CGCTGTCGCTGCTACTACTG |