| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143060 |
| Chromosome: | chromosome 3 |
| Location: | 238707 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144404 | (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAGGACATGATACGGCGCCAGAAGAGC |
| Internal bar code: | TCCTCCAGGCCGCCTGCTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 26.8115 |
| LEAP-Seq n confirming: | 4244 |
| LEAP-Seq n nonconfirming: | 11585 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAGTTGTCACCTTGAGT |
| Suggested primer 2: | GAGGCTCGTAAAACACCTGC |