| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143080 |
| Chromosome: | chromosome 5 |
| Location: | 2933441 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238250 | BLZ2,BZIP1 | (1 of 1) K04450 - cyclic AMP-dependent transcription factor ATF-2 (ATF2, CREBP1); Basic leucine zipper1 transcription factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGATCCTCGGAAAACAGTTCATCGTCCA |
| Internal bar code: | TCTCCGTGCACGCACGCGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 99.7081 |
| LEAP-Seq n confirming: | 3416 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGAAAGGAAAAGGCAACC |
| Suggested primer 2: | CAGCCGTGCTCTTAGGAAAC |