| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143100 |
| Chromosome: | chromosome 2 |
| Location: | 3259135 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095079 | (1 of 2) IPR000104//IPR011705 - Antifreeze protein, type I // BTB/Kelch-associated | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTACGGACATCTCGTTTGTCTGCGGTT |
| Internal bar code: | ATCCACGGGGTCGCGTGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 99.9202 |
| LEAP-Seq n confirming: | 5006 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGAGGTCCAACAGCACCG |
| Suggested primer 2: | AGGCTCTACGCAAGCAGAAG |