| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143129 |
| Chromosome: | chromosome 17 |
| Location: | 2293539 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713900 | LST8 | TOR kinase binding protein; (1 of 1) K08266 - G protein beta subunit-like (GBL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTATCTACTCCCCCTCATCCACCAGTCC |
| Internal bar code: | CTAGATGGCTTGGCAGGCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 90.5433 |
| LEAP-Seq n confirming: | 1800 |
| LEAP-Seq n nonconfirming: | 188 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGTCCGTCGAAGTTTG |
| Suggested primer 2: | GTTAGGCCACATGGTGCTTT |