| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143150 |
| Chromosome: | chromosome 11 |
| Location: | 2052584 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468600 | Hypothetical protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCGTGAATGAATACTTGATGCTGATGA |
| Internal bar code: | ATCTGAGGTTAAATCTGGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 99.542 |
| LEAP-Seq n confirming: | 652 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTTAATTGCTAAGCGCC |
| Suggested primer 2: | ATCGGCTGAATGTCCTATGC |