| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143152 |
| Chromosome: | chromosome 10 |
| Location: | 4523311 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452150 | (1 of 100) IPR001810 - F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGCTATAGGTGTCGGTTTAAGCAGATG |
| Internal bar code: | CCACAGTTTACTGGGACGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 99.7731 |
| LEAP-Seq n confirming: | 1759 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGCAAAGAGCAGGACTA |
| Suggested primer 2: | TTGTACTTTCTGCCGTTCCC |