Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.143159 |
Chromosome: | chromosome 10 |
Location: | 6293315 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g465050 | (1 of 1) PTHR19303//PTHR19303:SF285 - TRANSPOSON // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCTCCTCATCCTCCTCGTCGCCACTGC |
Internal bar code: | CAAAGGCGGGGTCCATTATCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 432 |
LEAP-Seq percent confirming: | 54.9287 |
LEAP-Seq n confirming: | 886 |
LEAP-Seq n nonconfirming: | 727 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGATTGCAATTGACTCGCTC |
Suggested primer 2: | GTCTCAGACGGTGCTCAACA |