| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143164 |
| Chromosome: | chromosome 5 |
| Location: | 1074363 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246150 | CYG13 | Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGTTGAACAAGGCCGACGATGGGCAAT |
| Internal bar code: | GGCGGGGATCAAGCCCTCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 942 |
| LEAP-Seq percent confirming: | 99.8953 |
| LEAP-Seq n confirming: | 6681 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGCCGGTGATAACAAT |
| Suggested primer 2: | ACATCCACCCAGTTGCTTTC |