| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143172 |
| Chromosome: | chromosome 2 |
| Location: | 1673201 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085750 | (1 of 8) PF13416 - Bacterial extracellular solute-binding protein (SBP_bac_8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCAGCCCGGACCTCAAAATCCGCCTCA |
| Internal bar code: | AAGACGACCGTTTTGATGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 994 |
| LEAP-Seq percent confirming: | 99.296 |
| LEAP-Seq n confirming: | 8039 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGAGAAAATGAGCGAGG |
| Suggested primer 2: | AGGAGGCAAACATTGGTGAC |