| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143173 |
| Chromosome: | chromosome 16 |
| Location: | 7420761 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686173 | (1 of 1) PTHR11071:SF276 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTAAGCTTGAATGACTCTGAGTAGTTTA |
| Internal bar code: | TGGAGGCATGATTAATCGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACACTGCTTTCTTCGCA |
| Suggested primer 2: | CCTCTCTCTCCAGCCCTTTT |