Insertion junction: LMJ.RY0402.143181_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.143181
Chromosome:chromosome 13
Location:86776
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g562501 SELD,SELD1 (1 of 1) 2.7.9.3 - Selenide, water dikinase / Selenophosphate synthetase; Selenophosphate synthetase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTCGGCAGCAGTAATGGGGAGTGATGCC
Internal bar code:CCGACAGCCGGGCACTCCGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:519
LEAP-Seq percent confirming:99.4396
LEAP-Seq n confirming:1597
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGTTCGTTTCTCGCTTCC
Suggested primer 2:AGACCCCAAACCAGACACTG