Insertion junction: LMJ.RY0402.143204_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.143204
Chromosome:chromosome 11
Location:1194759
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467691 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACGCCTCCGGAGGCGATGGCGCCACCCG
Internal bar code:TTGCGGCCGTTAAGCCAGGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:239
LEAP-Seq percent confirming:98.7342
LEAP-Seq n confirming:156
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCAGCTGACGACGGAGAC
Suggested primer 2:TCTGCTTACCCACACTGCTG